You need a myeppendorf account to use product registration. Combine any mix of mastercycler pro gradient s, mastercycler pro gradient, and mastercycler pro 384 to a network of up to 30 units and control the entire network with one computer. Rt 2 sybr green qpcr mastermixes are also suitable. Mastercycler ep realplex from eppendorf biocompare product. Thiamine deficiencymediated brain mitochondrial pathology. Mastercycler nexus termociclatori, pcr eppendorf italia. Each thermal cycle is equipped with highperformance. For steponesteponeplus systems, install software version 2. Real time pcr was performed using an rt 2 profiler pcr array system sabioscience qiagen, valencia, ca, usa with sybr green pcr master mix, according to manufacturers instructions, in a mastercycler ep realplex thermocycler eppendorf, hamburg, germany. Eppendorf training become an expert with our training program.
Mastercycler ep realplex from eppendorf selectscience. Orbital circular paths with a diameter of 3 mm and up to 3,000 rpm. The mastercycler performance plans offer a choice of depending on frequency of usage of your mastercycler ep realplex or environmental. Softwarefirmware revisions are made available at no charge through this web page so that all users can benefit from the latest. This mastermix should be used in qpcr instruments under standard cycling conditions. Connection of mastercycler ep realplex to a computer. Mastercycler x50 cyclers, pcr eppendorf international. The software does a great job of saving and archiving all of your data, making it almost impossible to lose or overwrite anything. Find online auctions and classified ads for eppendorf products and more on the labx marketplace. In combination with eppendorfs pcr tubes, pcr strips or divisible plates, the mastercycler nexus x2 will give you consistent and publishable results every day.
B a plot of copy number log versus c t value demonstrates high linearity. It has a small footprint 40cm x 24cm x 40cm, which should appeal to those working in small or overcrowded laboratories. The mastercycler nexus can accommodate 96well pcr plates, 0. Mastercycler nexus x2 cicladores, pcr eppendorf brasilien. Gmp, glp, gxp simplify method development with editors and feature sets hardware upgrades for ep motion improve daily lab work. We enable science by offering product choice, services, process excellence and our people make it happen. This eppendorf cyclemanager pro software allows control of up to 30 mastercycler pro with one computer. May 14, 2008 both realplex machines use the same software interface, and its a great one, which is a piece of cake to use. Eppendorf limited product warranty mastercycler ep. Five different colors can help organize your workflow. Shop online for a wide selection of eppendorf mastercycler pro pcr system proprietary tect technology reduces evaporation to a minimum, improving reproducibility eppendorf mastercycler pro pcr system.
The differences between each system include the number of. Most samples are efficiently mixed within less than a minute independent of vessel geometry or sample properties. The mastercycler nexus thermal cycler brings reliability and ease of use along with low energy consumptionthermal cyclers are used to amplify segments of dna via the polymerase chain reaction. Thiamine deficiencymediated brain mitochondrial pathology in. Thats when you need high quality pcr cyclers and consumables to eliminate unwanted variables from your assays. Mastercycler ep realplex realtime pcr system eppendorf. Tips and tricks helpful infographics to support your daily work. Mastercycler nexus x2 cyclers, pcr eppendorf norge. Mastercycler nexus x2 cyclers, pcr eppendorf south pacific.
Also used to facilitate other temperaturesensitive reactions, the devices can complete restriction enzyme digestion or rapid diagnostics. The mastercycler ep realplex 2 gradient thermocycler eppendorf is a realtime pcr system with high ramping speeds and short detection times. Rely on the cfx connect system to perform precise twotarget pcr. Avantor is a trusted global partner to customers and suppliers in the life science, advanced technology and applied materials industries. Biorad bundles fcs express flow cytometry analysis software with the ze5 cell analyzer cancer researchers. Mastercycler nexus cyclers, pcr eppendorf south pacific.
Indicates kit should work, but has not been tested on this instrument. Mastercycler nexus x2 cyclers, termocicladores eppendorf. Mit dem mastercycler nexus x2 konnen sie zwei vollig unabhangige protokolle gleichzeitig ausfuhren. Lost software eppendorfmastercycler ep realplex questions. A freely programmable temperature gradient range of up to 20c enables the optimization not only of the annealing temperatu. Select the destination for the file and enter a filename. A standard 40 cycle taqman assay can be run in approximately 80 minutes, including data analysis and report generation. Eppendorf mastercycler ep realplex instrument setup instructions for rt 2 profiler pcr arrays important points before starting please read the handbook supplied with the rt 2 profiler pcr array, paying careful attention to the safety information and important notes sections, before beginning this procedure. The quantstudio 3 system can be used for limited multiplexing. We understand every lab is unique and will work with you to develop a seamless and integrated online procurement strategy. Eppendorf licenses thermal gradient ip to takara bio. Switch on mastercycler ep realplex and wait until the led on the top switches to green.
Promotions learn about how you can save on eppendorf products with our current promotions. Mastercycler nexus x2 cyclers, pcr eppendorf united kingdom. Mastercycler service get your service for absolute confidence. Mastercycler nexus x2 cyclers, pcr eppendorf nederland. Mastercycler ep realplex software setup for the quantifast probe. The mastercycler gradient sets the standard for optimization of pcr reaction conditions. Mastercycler mastercycler gradient operating manual.
Extremely fast temperature ramping, short detection times and intuitive assay programming translate to huge timesavings so that more. Mastercycler ep with epblue eppendorf cerajodhmord. The name of the program executable file is realplex. Check back regularly for our monthly promos and new ways to save. The eppendorf mastercycler pro offers ultimate flexibility for your pcr applications. Copy and save results to special software tools for relative quantification 104. Eppendorf mastercycler ep realplex instrument setup instructions for.
Generates excellent mixing performance, even for challenging samples. The eppendorf mastercycler and mastercycler gradient are pcr devices for all molecular biological and biochemical laboratories in the. Jun 04, 2012 eppendorfs mastercycler ep realplex is an instrument that exceeds the demand for rapid, accurate and reliable results. However, it can be difficult when you want to do something that you. The eppendorf mastercycler ep 384 thermal cycler system brings you reliable and fast gradient pcr cycling for the best in optimized reaction conditions. Our mastercycler pro has a special lid to avoid evaporation and superior block homogeneity to achieve consistent results, despite the well being used. Cfx96 touch realtime pcr detection system biorecherche. Eppendorf said this week that it has licensed patents covering gradient technology for thermal cyclers to takara bio the licensed patents include us nos. In our laboratory, we use it routinely for quantitative mrna expression studies, verification of microarray results and pathogen mycoplasma detection in eukaryotic systems. Mastercycler x50 cycler, pcr eppendorf deutschland. Mastercycler ep realplex from eppendorf biocompare. The most popular version of this product among our users is 2. The mastercycler nexus pcr cycler provides scientists with flexibility in many forms.
The expert design and wetlab validation of all primepcr assays ensure optimal. The mastercycler x50 family consist of units with a userfriendly visionize touchscreen interface or eco units which are externally controlled. Eppendorf s mastercycler ep realplex is an instrument that exceeds the demand for rapid, accurate and reliable results. The eppendorf mastercycler ep gradient s thermal cycler features electronic sample protection or esp, a unique system designed to ensure the prevention of early heating before the cycle is set to begin and to minimize unspecific annealing. In this supplement, you will only find the features listed, which. Hence, the time it takes to complete an assay is decreased. We operate in more than 30 countries and deliver an extensive portfolio of mission critical products, services and solutions. Eppendorf enewsletter subscribe to our email newsletter and you wont miss a thing. The mastecycler pro is unparalleled in its ability to fulfill requirements. The 96 well aluminium block can accomodate several standard vessels types from 0. Modified slip cover light transmissive cover for optical openings in heated platen assembly, us patent pending.
Eppendorf mastercycler ep gradient thermal cycler 96 well. The contents of the delive ry package depend on the variant of your mastercycler pro. The software automatically saves each assay, which provides a back up for. Register a myeppendorf device using the eppendorf app using the eppendorf app is the simplest and easiest way to register your eppendorf device. It is a compact, easy to use and flexible platform. The new mastercycler x50 is the elegant synthesis of speed, flexibility and pcr optimization for research pcr applications and standardization for routine pcr applications such as food testing etc. Realtime pcr master mixes and instrument compatibility. Grazie al concetto flexlid, il cycler puo utilizzare tutti i tipi di materiali di consumo pcr, a partire dai bassi volumi fino alle provette pcr da 0,5 ml. The qpcr was performed on a mastercycler ep realplex thermocycler eppendorf, westbury, ny. The mastercycler nexus x2 is eppendorfs solution for busy pcr labs. Product description the mastercycler nexus x2 gives you the ability to run two totally independent protocols at the same time.
Like the realplex 2, this machine is small, solid and very quiet. The reaction efficiencies obtained for the presented assays, including the. As every member of the mastercycler nexus family, the mastercycler nexus x2 can be combined with all other family members in a network of up to 3 units. Mastercycler nexus x2 cycler, pcr eppendorf austria. Cfx384 touch realtime pcr detection system biorecherche. Two totally independent protocols can be run on the two blocks.
Rt 2 sybr green qpcr mastermixes are highly suited for realtime pcr applications using sybr green, and are required for guaranteed qpcr performance of the rt 2 qpcr primer assays, rt 2 profiler pcr arrays, rt 2 lncrna qpcr assays, and rt 2 lncrna pcr arrays. This is eppendorfs bigbrother qpcr platform, which in many ways is similar to the realplex 2. Leds generate a blue light at a wavelength of 470 nm, which can excite nearly all fluorophores used in realtime pcr. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips. Heparin spiked in from 200 to 3,200 pgl caused the 17. Mastercycler ep realplex is compact in size, so it can fit in virtually any lab o escolhido hannah howell download pdf, no matter how limited the powered by sunshop shopping cart software. The machine features, like some other mastercyler devices from eppendorf, a special 96well heating block, which enables rapid thermal cycling. Configuration of the database tool is only accessible for an administrator. Comprehensive yet intuitive software paired with recent advances in realtime pcr technology make the mastercycler ep realplex a critical contribution to modern science and research. Learn more about thermal cyclers, mastercycler ep realplex. Low volume realtime pcr on the mastercycler ep realplex. Small, fast and flexiblethe mastercycler ep realplex realtime pcr system meets all the latest requirements of quantitative pcr applications. Cfx manager software can independently run four instruments, including a combination of cfx96 touch, cfx96 touch deep well, or cfx384 touch. With less than 40 dba noise emission, the nexus is whisper quiet, while also being energy efficient.
The mixmate provides enough mixing power to handle also challenging mixing tasks. Le mastercycler nexus offre aux scientifiques une flexibilite dans diverses formes. This eppendorf mastercycler ep gradient thermal cycler was tested by our service team multiple times to ensure system is working to factory specification. For completion, the following features concerning operation of the mastercycler ep realplex are not accessible to restricted users. In a normal working day in our laboratory, the realplex 2 can be run four to five times, depending upon assay type.
Both are operated by the same realplex software, and both are equipped with an led array to excite the. Eppendorf mastercycler ep gradient s thermal cycler w 96well universal block. Cfx maestro software works seamlessly with primepcr assays and panels. This allows two pcr users in the lab to start and end their pcr reactions whenever they want. Mastercycler pro upgraded to a mastercycler ep realplex requires a realtime thermal cycler license under appleras united states patent no.
Open the eppendorf app which is linked to your myeppendorf account and click on the product registration panel. The mastercycler x50 family boasts ramp rates of up to 10 cs and is the ideal cycler family for routine applications as well as advanced research in molecular biology. Development and interlaboratory validation of unlabeled. Set oncoprotein accumulation regulates transcription. Temperature control is carried out with peltier elements, which enable extremely rapid changes in. Extremely fast temperature ramping, short detection. Trying to fix another equipment i foun a realplex 4 stopped because someone lost the pc software. Mastercycler ep realplex software setup for the quantifast. Eppendorf mastercycler ep realplex instrument setup instructions for rt2 profiler pcr arrays. The gradient temperatures of this mastercycler thermocycler is freely programmable over 12 rows up to 20c, allowing for annealing temperature and all steps of a pcr protocol.
388 1233 1129 984 1391 502 829 960 190 1337 1134 1564 994 76 368 160 7 1033 554 811 81 852 1325 1109 1225 577 470 325 1023 1031 485 50 88 1240 562 1150 1430 523 317 909